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2010 2nd Sem Final Study Guide

2011 SHSID G10 S Biology Course Study Guide for 2nd Semester Final Exam 10S Biology Exam Date: June 16, 2011 (Thursday) Range: 10% (10.1, 10.2, 10.3, 11.1, 11.2, 11.3) + 90% (12.1, 12.2, 12.3, 13.1, 13.2, 13.3, 13.4) Chapters 10 to 12: See the previous review sheets.

Section 13.1 1. Key terms and concepts: * traits *selective breeding * hybridization * inbreeding *mutation *polyploid *aneuploid 2. What are the applications of selective breeding? What are some examples? 3. What is the difference between hybridization and inbreeding? What are some examples? 4. Summarize all kinds of mutations. What are the similarities and differences among the different types? Section 13.2 1. Key terms and concepts: * restriction enzyme * recombinant DNA * gel electrophoresis * DNA replication * polymerase chain reaction (PCR)

2. Know the target and the mechanism of restriction enzymes. 3. Know how gel electrophoresis and PCR work. 4. In gel electrophoresis, do small molecules or large molecules move more quickly? Why? 5. In gel electrophoresis, where is the negative charge and where is the positive charge? Why? Section 13.3 1. Key terms and concepts: * cell transformation * plasmid * genetic marker * antibiotics 2. Be familiar with the experiments depicted in the diagrams this section. Know what the researchers did in these experiments, what observations they made, and what conclusions they drew. 3. What goes wrong in cancer, and what things can cause cancer? Section 13.4 1. Key terms and concepts: * transgenic bacteria * transgenic plant * transgenic animal * genetic engineering * cloning 2. What are the main ways of making transgenic plants and transgenic animals? 3. How do transgenic plants and animals benefit our life? 4. What were the three sheep that contributed to making Dolly? Among them, which is the real mother of Dolly? Why? 5. What are the ethical concerns pertaining to transgenic plants, transgenic animals, and cloning strategies?



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